Part:BBa_K1486014:Design

IFP[1]-CpxR under pBAD

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1481
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal PstI site found at 1481
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  Illegal XhoI site found at 1718
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1481
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1481
  Illegal AgeI site found at 979
  Illegal AgeI site found at 2129
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

IFP1 was fused to the N or C terminal of the CpxR. A flexible linker was used to fuse both proteins. WARNING: It is also to be noted that basic parts are submitted with a start codon and a stop codon. To make a composite part, START and STOP codons of the basics parts may have been removed, as we only want a START codon at the beginning of a cds and a STOP codon at the end of a cds. You should consider it when looking at the part sequence.

Source

We received Michnick lab IFP1 and IFP2 sequences from [http://www.addgene.org/52899/ addgene].

References

Michnick, S., Tchekanda, E., & Sivanesan, D. (2014, April 20). [http://www.nature.com/nmeth/journal/v11/n6/full/nmeth.2934.html An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.] Nature Methods, 6-6.